Examination of Lactic Acid Bacteria’s Ability from Fish Intestinal Tract in Deconjugation Bile Salt

The study was carried out to determine the ability of lactic acid bacteria (LAB) isolated from fish intestine to do deconjugation of bile salt. Bile salt deconjugation was shown by the production of cholic acid. This experiment was consisted of six treatments with five replicates. The treatments in thus experiment were addition of medium with bile salt equal to 0, 1.0, 0.2, 0.3, 0.4, and 0.5%. Lactic acid bacteria was grown in medium at 39C for 20 h. Variables observed were density per h., pH, and cholic acid produced in the end of fermentation process. The data obtained were analyzed using variance analysis with one way of Completely Randomized Design (CRD), differences between mean values of each variable were tested by Duncan's Multiple Range Test (DMRT). The changes of density show that LAB was able to grow in medium that added bile salt until level of 0.5%. Nevertheless, the addition bile salt in the medium decreased the growth rate of LAB during exponential phase. The production of cholic acid were 0.005, 0.087, 0.175, 0.211, 0.202, 0.150 μmol/ml, while the pH values were 4.6, 4.7, 4.92, 5.00, 5.05 and 5.15 respectively for the addition of 0, 0.1, until 0.2% in added 0.5% the production cholic acid decrease significantly. It could be concluded that LAB isolated from fish intestine was able to do deconjugation of the bile salt until level 0.4%.